Have you ever been in a clinic with your head up and mouth open for a regular buccal swab test but innocently having no idea whether your sample tube would work or not?
All right, first of all everything depends on the selective flocked swab on your doctor’s hand. And don’t get us started on cotton sticks! That’s a whole new story. The results of the following comparison experiment might speak the truth louder to those who lack a comprehensive understanding of this topic.
At the very beginning, the objective of this experiment is to investigate the effective rates of DNA sample collection and releasing from flocked swabs [tagged group F] and traditional cotton swabs [tagged group C]. Here Mantacc’s flocked swab was included in the experiment as a professional representative which has already played an innovative and responsible role in the global market.
How to prove the advantages of a flocked swab compared to a traditional cotton swab？
- For a pre-collection preparation, 8 sample tubes of equal fresh whole blood with anticoagulant were diluted with multiple deionized water in the sequence of 2, 4, 8, 16, 32, 64, 128, 256 times under the same environment.
- Scientists took a 2 μl required solution from each tube and made the group F [8 flocked swabs] and group C [8 traditional cotton swabs] absorbing it separately. When all the dry samples had completed the collection step, the Chelex-100 was used extract their DNA absorbed.
- The Identifier Plus kit was adopted to fulfill the necessary amplifications. Eventually, a gene analyzer type 3500XL performed the comparison detection of the DNA extraction rates among stepwise concentration samples of group F and group C respectively.
After the completion of the main experiment procedures, the analytical result was brought out to the comparative data foundation. As the variable observation showed, there was merely a slight difference among samples under 16-time-diluted solutions. On the contrary, the group F conducted a conspicuously higher absorption and releasing rate than the group C, which used solution samples diluted from 16 to 64 times. From the perspective of the peak height according to the detection result, the results achieved by group F were twice as high as group C’s ones at all distinct concentration solution samples. It is also worth mentioning that the group F showed a significantly better equilibrium than the group C among the concentration samples from 16 to 64 times.
What’s the real point of these discoveries？
According to the analytical variation, scientists proved that flocked swabs can achieve more effective rates of DNA sample collection and releasing than traditional cotton swabs, especially under a low-concentration trace blood sample testing. As a matter of fact, medical-level sample collection products should accommodate a whole variety of conditions. For example, Mantacc’s series are suitable for a wide range of sensitive downstream applications, including PCR, qPCR, sequencing, SNP analysis, microarrays, RFLP, and Southern Blot Analysis.
What else should you know about sample collection？
Remember the scene we mentioned at the very beginning of this article? It is undeniable that sample collection functions as an important bridge connecting science and daily life. It helps us to dig deeper in the knowledge we have about science and unveil the relationship between complicated objective facts. The results achieved in comparative effectiveness demonstrate that the fine flocking materials not only contribute to a more user-friendly experience. They can also conduct the scientific sample collection and the releasing of DNA, saliva, or other elements more reassuring. For example, your doctor might offer you a strange prescription based on your oral swab test results, which you could not decode by yourself at home.
In addition, several laboratory studies or pathological analysis require more professional testing equipment. The storage and transportation devices should be filled with viral transport media like Amies transport medium, Cary Blair transport media, or Stuart transport medium, which reach the standards of both stability and sealability.
Considering the welfare of the next generation, flocking is also globally recognized as one of the most environment-friendly material choices for creative swab experts like Mantacc. It is an organizations’ responsibility to make the potential demand of the global market more sustainable. In a word, a cotton swab can be used for sampling, but it is not good enough. A flocked swab is a much better option. So why not switching to the latter?